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We present an automatic approach for 3D segmentation of mouse embryonic stem cell nuclei based on level set active contours. Due to the specific properties of these cells, standard methods for cell nucleus segmentation and splitting of cell clusters cannot be applied. Our segmentation approach combines information from two different channels which represent the nuclear region and the nuclear membrane...
Carbon nanotubes (CNTs) have been widely investigated as one of the most promising nanomaterials in biomedical applications. Functionalization of CNTs with poly(ethylene glycol) diamine (PEG) is a recognized methodology with good solubility and biocompatibility. In this study, PEG conjugated (PEGylated) multi-walled CNTs (MWCNTs) were prepared and labeled with fluorophore FITC. Using fluorophore-conjugated...
The targeting moiety (B-subunit) of the Shiga toxin (STxB) is a very potent ligand for the glycolipid Gb3, which is expressed in ovarian, colorectal and breast carcinomas. It is also present on endothelial cells of tumor neovascularization. This study demonstrates the use of STxB for targeting microbubbles onto cancerous cells. STxB-functionalized microbubbles and biotinylated controls were incubated...
To study the effect of qinglongyi polysaccharide on cation concentration in erythrocyte of S180 mice A tumor mouse model was developed, and the mice were treated with high, medium, and low doses of qinglongyi polysaccharide through abdominal injection for 7 d. Erythrocytes were collected and erythrocyte suspensions were prepared. Change of Ca2+ concentration in the erythrocytes of S180 mice was measured...
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