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Endocytosis/recycling and exocytosis are mechanisms conserved through evolution allowing cells to communicate with their external medium. In order to study these dynamic processes, the present work proposes a patch-based method for detecting recycling or exocytotic events at the Plasma membrane in fast TIRF microscopy combined with the computation of normalized temporal representations of those events...
This paper presents a novel computer vision system for automated identification of vesicle-plasma membrane fusion events in image sequences obtained from Total Internal Reflection Fluorescence (TIRF) microscopes. Identification of such events is important in order to better understand the process of exocytosis in cells. Manual analysis of thousands of images is painstakingly slow and subjective since...
A wide range of proteins viewed using xFP probes in fluorescence video-microscopy shows a cytosolic state diffusing slowly and a membrane state eventually moving as vesicles or small tubules along the cell cytoskeleton network. Due to the property of fluorescence, the measured intensity is the sum of the contributions of these two components. In this study we perform an evaluation of different methods...
Recent approaches to record and analyze skeletal muscle elementary calcium release events (ECRE) in three dimensions (XYT) were restricted to short time series and small areas of interest. Here, we present a combined method for XYT analysis of ECRE using high-speed multifocal multiphoton calcium imaging and a wavelet based automated denoising and detection algorithm. Images were analyzed with the...
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