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In order to investigate the effects of electric pulses on cancer cells, HepG2 and L02 were exposed to electric pulses (1000V/cm, 100μs, 1Hz) for different time (8s, 15s, 30s, 60s). Annexin-V-FITC Kit and Flow cytometry were used to study the apoptosis of treated cells. The results showed that the electric pulses of 1000V/cm, 100μs, 1Hz for 8s couldn't induce tumor cells apoptosis. Apoptosis was observed...
In this work, a micro electroendocytosis (EED) device was fabricated by MEMS technology to investigate the EED of mammalian cells. The corresponding endocytotic vesicles at the single-cell level were visualized using digital fluorescence video microscopy. The EED process under different electrical and biological parameters was studied in detail. The critical EED electric field strength for the HeLa...
We present a new microscopy technique allowing electric field mapping in molecular media with micrometer resolution : electro-optical microscopy. We apply it to polymer devices and local measurements of transmembranar potentials in biomimetic membranes.
Summary form only given. Experimental studies have been conducted to investigate the plasma membrane response of HL-60 human promyelocytic leukemia cells to pulsed electric fields (PEFs) with pulse widths ranging from 125 nanoseconds to 1 millisecond. Both single and trains of monopolar PEFs were delivered to HL-60 cells suspended in micro-fabricated micro-cuvettes. The cells were monitored in real-time...
Recent approaches to record and analyze skeletal muscle elementary calcium release events (ECRE) in three dimensions (XYT) were restricted to short time series and small areas of interest. Here, we present a combined method for XYT analysis of ECRE using high-speed multifocal multiphoton calcium imaging and a wavelet based automated denoising and detection algorithm. Images were analyzed with the...
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