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We present an automatic approach for 3D segmentation of mouse embryonic stem cell nuclei based on level set active contours. Due to the specific properties of these cells, standard methods for cell nucleus segmentation and splitting of cell clusters cannot be applied. Our segmentation approach combines information from two different channels which represent the nuclear region and the nuclear membrane...
Recent approaches to record and analyze skeletal muscle elementary calcium release events (ECRE) in three dimensions (XYT) were restricted to short time series and small areas of interest. Here, we present a combined method for XYT analysis of ECRE using high-speed multifocal multiphoton calcium imaging and a wavelet based automated denoising and detection algorithm. Images were analyzed with the...
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