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Cancer marker proteins have been electrophoretically concentrated and then separated in a microfluidic device. On‐chip preconcentration was achieved using an ion‐permeable membrane, consisting of acrylamide, N,N′‐methylene‐bisacrylamide and 2‐(acrylamido)‐2‐methylpropanesulfonate. This negatively charged membrane was photopolymerized in the microdevice near the injection intersection. Anionic proteins...
Capillary electrophoresis amperometric detection (CE–AD) microchip was fabricated for separation and detection of DNA fragments. The chip was fabricated on glass substrate and microchannels were laid in PDMS mold. The capillary was filled with 5% polyacrylamide gel and separation of DNA fragments of different molecular weight was achieved by application of 100V DC potential across sample and waste...
There has been significant interest in the adaptation of lab on a chip devices for the separation and detection of chemical and biological toxins. Potential toxic and/or hazardous analytes of concern in our program include BWA protein toxins,e.g., SEB and ricin, ingestible CW toxins, e.g. alkaloids and rat poisons, and nitroaromatic explosives indicative of IED's. Discussion will center around our...
This paper presents an experimental and numerical investigation into the use of high-resolution injection techniques to separate DNA fragments within electrophoresis microchips. The study addresses the principal material transport mechanisms such as electrokinetic migration, fluid flow, diffusion, variable-volume injection methods, and gives detail analyses to the double-L injection technique, which...
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