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A methodology for NMR analysis of low nanomole amounts of oligosaccharides fractionated by analytical HPAEC is presented. Arabinoxylan derived oligosaccharides purified by HPAEC-PAD on an analytical column, by single injections, were analyzed with nano-probe NMR and MALDI-TOF MS to provide full structural assignment. The NMR data were obtained with a 500 MHz NMR spectrometer equipped with a 1...
Non-starch polysaccharides (NSP) content, partially water-soluble and high-molecular-weight polymers, are related to beer viscosity. Thus, arabinoxylan alone, or synergistically with β-glucan, may be involved in some of the undesirable effects in the brewing process, such as influencing the rate of wort separation. Three malting barley samples were studied to determine glycosyl linkage composition...
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