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The mechanisms of signal transduction of c-jun induction by hydrogen peroxide are elucidated in NIH3T3 cells by using trapping agents of hydroxyl free radical or inhibitors of various protein kinases. Pre-treatment of the cell with hydroxyl radical scavenger dimethyl sulfoxide (DMSO) abolishes the H 2 O 2 -induced c-jun expression. Hydroxyl radical generation can be detected and quantified...
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