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Batch suspension cultures of Lucerne (Medicago media cv Rambler), established directly from surface-sterilized leaflet explants, were subcultured into media of progressively higher salinity to produce a cell line tolerant to 400 mol/m 3 NaCl. After these cells were grown for eight passages (28 days each) on NaCl-free medium, the cells were still more salt-tolerant than non-selected cells when...
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