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In this paper, we present a fluorescence collection enhancement method for multiphoton microscopy by a quarter ellipsoidal reflector (QER). It is proven to be compatible with commercially available two-photon microscopes without significant customization. The structure and working principle of the QER is demonstrated. A QER was fabricated to collect most backscattered photons not collected by an objective...
We propose a holographic-laser-drawing volumetric display using a computer-generated hologram displayed on a liquid crystal spatial light modulator and multilayer fluorescent screen. The holographic-laser-drawing technique has enabled three things; (i) increasing the number of voxels of the volumetric graphics per unit time; (ii) increasing the total input energy to the volumetric display because...
Linear and nonlinear optical imaging systems are currently being miniaturized to fit endoscopic size requirements of a diameter less than 2 mm. These systems are used for deep imaging and optical biopsy. We report our progress in the development of a multimodal microendoscope based on Gradient Index (GRIN) lenses and double clad fiber (DCF) for femtosecond pulse delivery and signal collection. Four...
We present recent advances on scanning endomicroscopy technologies which can perform nonlinear optical imaging with an ultracompact fiber-optic platform of a 2mm diameter. Two-photon fluorescence and SHG imaging of unstained biological tissues will be reported.
Light is strictly connected with life, and its presence is fundamental for any living environment. Thus, many biological mechanisms are related to light interaction or can be evaluated through processes involving energy exchange with photons. Optics has always been a precious tool to evaluate molecular and cellular mechanisms, but the discovery of lasers opened new pathways of interactions of light...
We describe an optical imaging methodology applicable to the detection of a fluorescent agent below the surface of turbid media with lifetime property extraction. The method exploits the collimation detection capabilities of an angular filter array to form a projection image of a fluorophore embedded within the tissue-mimicking phantom. A femto-second pulsed laser was used to illuminate the tissue...
Fluorescence mediated tomography allows quantitative, three-dimensional imaging of optical reporter probes in whole animals and is therefore emerging as a powerful molecular imaging tool. The achievable image quality in fluorescence tomography is limited by the high-degree of light scatter in biological tissue. Time-gated detection of early-arriving and therefore minimally-scattered photons transmitted...
We propose and develop a nanoprobe-based technique for characterizing femtosecond laser pulses. A preliminary demonstration based on the measurement of the interferometric autocorrelation trace through two-photon fluorescence from a nonlinear nanoprobe is reported.
Optical storage inside a human fingernail is performed. A bit is recorded by irradiating of a femtosecond laser pulse and the bit is read by use of increased fluorescence at the formed bit.
We demonstrate the first use of femtosecond laser pulses for serial histology. Successive iterations of multiphoton imaging and ablation provide diffraction-limited volumetric data that is used to reconstruct the architectonics of labeled cells or microvasculature.
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