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Imaging in awake, behaving animals is an emerging field that offers the advantage of being able to study physiological processes and structures in a more natural state than what is possible in tissue slices or even in anesthetized animals. To date, most imaging in awake animals has used optical fiber bundles or electrical cables to transfer signals to traditional imaging-system components. However,...
HiLo microscopy is a widefield fluorescence imaging technique that provides depth discrimination by combining two images, one with non-uniform illumination and one with uniform illumination. We discuss the theory of this technique and a variety of practical implementations in brain-tissue imaging and fluorescence endomicroscopy.
Optical recording of the activity of hundreds of individual neurons simultaneously within the functioning brain is now possible with calcium sensitive dyes. This offers a major advance over the limitations of single-unit recording with arrays of microelectrodes, or with functional MRI. However, the analysis of optical activity to understand neuronal interactions and circuitry underlying physiological...
Multimodal nonlinear optical microscopy that integrates coherent anti-Stokes Raman scattering, second harmonic generation, and two-photon excited fluorescence is becoming an essential tool for tissue biology. Applications to white matter, atherosclerosis, obesity, and cancer are demonstrated.
In computer vision, shape recovery is a classic problem. The shape from shading problem aims to reconstruct a 3D shape from one or more intensity images. Inspection of solder joint is a critical step in the assembly of PCB (Printed Circuit Board). So, non-destructive inspection technology has been widely used in a production of PCB. In automated optical inspection (AOI) field 2D inspection appears...
Optical imaging is a powerful technique to map brain function in animals. In this study, we consider in vivo optical imaging of the murine olfactory bulb, using an intrinsic signal and a genetically expressed activity reporter fluorescent protein (synaptopHfluorin). The aim is to detect odor-evoked activations that occur in small spherical structures of the olfactory bulb called glomeruli. We propose...
We demonstrate the first use of femtosecond laser pulses for serial histology. Successive iterations of multiphoton imaging and ablation provide diffraction-limited volumetric data that is used to reconstruct the architectonics of labeled cells or microvasculature.
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