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Optical Coherence Tomography (OCT) is a well known technique for creating non-invasive, high resolution images of biological microstructure. OCT has matured into animportant imaging modality since its invention in 1991. The popularity of the method lies in the numerous advantages that it offers high detection sensitivity allowing fast imaging speed, andhigh spatial resolution. In this paper we propose...
A new optical platform for the interrogation of a polymethylmethacrylate (PMMA) multichannel array for chemical and biochemical parameters is described. It consists of a plastic chip formed by two pieces of PMMA properly shaped in order to obtain different microchannels, 500 mum in width and 400 mum in height. A fluorescent sensing layer is immobilized on the internal wall of the microchannel. The...
This article presents the fluorescence lifetime characterization of haematoxylin and eosin (H&E)-stained cervical tissue using a wide-field time-domain fluorescence lifetime imaging microscope (FLIM). The transient response of epithelial fluorescence was investigated and approximated using a bi-exponential model. It was found that the fluorescence lifetimes of eosin can be correlated to the tissue...
Frequency domain fluorescence technique forms a strong platform for many sensitive biomedical imaging applications. In this paper, detection of human genomic DNA sample (in capillary tube) fluorescence was carried out with frequency-domain (FD) imaging technique. This sensitive imaging technique may provide a cost effective DNA fluorescence detection by minimizing the sample volume. Also, initial...
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