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The fluorescence-based integrated chemical and enzyme sensor arrays on which two kinds of 10 μm i.d. sensor spots were arranged alternately on one chip, were developed by using microcontact printing. FITC, ruthenium complex and europium tetracycline was stamped onto a DLC sputtered glass slides for pH, oxygen and hydrogen peroxide sensors, respectively. After printing of oxygen sensor spots, pH sensor...
It is known that the dynamic fluctuations in protein structures are essential for their biological functions, including channel gating, allosteric interactions, signal transduction, recognition dynamics and enzymatic catalysis. Understanding the functional mechanisms of such proteins requires the identification of the collective atomic motions and how these motions relate to the biological function...
A fiber optic bio-sniffer (biochemical gas sensor) for alcohol gas monitoring with high sensitivity and high selectivity was fabricated and tested. The bio-sniffer is a gas sensor that uses molecular recognition of enzyme to improve selectivity. Usually, enzyme loses activity in the gas phase. Applying a flow-cell with a gas-intake window to the sensing probe, enzyme immobilized at the sensing region...
The development of high-sensitivity and specificity probes that lack the intrinsic limitation of organic fluorescent dyes and sensitizers is of considerable interest in many areas of research, from cellular biology to molecular imaging and diagnostics. Quantum dots (QDs) are stable, bright fluorophores that can give high quantum yields, narrow emission bands, intense absorbance, very large Stokes...
Micro- and nanofabrication has allowed the creation of ultra-sensitive, miniaturized, and inexpensive biosensors. These devices generally utilize chemical or biological receptors which recognize a particular compound of interest and transduce this recognition event into a measurable signal. Recent advances in RNA and DNA synthesis have enabled the use of aptamers, which are in-vitro generated oligonucleotides...
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