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Indirect immunofluorescence (IIF) with HEp-2 cells has been used to detect antinuclear auto-antibodies (ANA) for diagnosing systemic autoimmune diseases. The aim of this study is to develop an automatic scheme to identify the fluorescence pattern of HEp-2 cell in the IIF images. By using the previously proposed two-staged segmentation method, the similarity-based watershed algorithm with marker techniques...
Autofluorescence bronchoscopy (AFB) has been utilized over the past decade, proving to be a powerful tool for the detection and localization of premalignant and malignant lesions of the airways. AFB is, however, characterized by low specificity and a high rate of false positive findings (FPFs). The majority of FPFs are due to inflammations, as they often fluoresce at the same wavelengths with cancer...
This paper outlines the role of multiphoton microscopy and optical nanoscopy in the nanosciences especially in the applicative areas of nano-characterization and nano-fabrication utilizing light-matter interactions.
We demonstrate a continuously electronically tunable (435-1150 nm) ultrafast source for fluorescence imaging applications that is derived from a visible supercontinuum generated by injecting infrared femtosecond pulses in to a microstructured fibre. We demonstrate this source applied to confocal and wide-field microscopy, as well as multiwell-plate imaging. We also report first application of a tunable...
Complex networks underlie the very foundation of neuroscience and the mammalian nervous system, yet high throughput computationally intensive measurements of molecular signaling between large groups of cells and the extraction of statistically significant quantitative information about the underlying network structure is not possible given current techniques. We have developed novel computational...
For real-time imaging of the transmembrane voltage of Jurkat cells, exposed to nanosecond pulsed electric fields, the cells were stained with a voltage sensitive membrane dye (VSD) and illuminated with a 4.8 ns long dye-laser pulse at various time during the electric field pulse. The stained cells were located in a 100 mum stainless steel electrode arrangement mounted at the stage of an inverted microscope...
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