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Observation and manipulation of a single DNA molecule in real time have been applied for gene analyses and novel biochemical analyses of DNA-protein interactions. The DNA molecule in solution frequently changes its position and its shape due to Brownian motion, therefore precise handling technique is required. At the same time, miniaturized chemical reaction systems such as micro total analysis systems...
A fibre-optic biochemical gas sensor (bio-sniffer) for gaseous formaldehyde (FA) was developed and applied for on-site assessment of FA detoxification. The bio-sniffer measures FA as a fluorescence of reduced nicoti-namide adenine dinucleotide (NADH) which is the product of formaldehyde dehydrogenase reaction. The detection limit of the bio-sniffer for gaseous FA was 2.5 ppb, which is enough lower...
Recently, there has been an increasing interest to propose computational approaches based on image processing to automate the comparison of spatial gene expression patterns contained in transgenic embryo images. The first step is typically to classify the images between lateral and dorsal/ventral views, and then to align them along the anterior and posterior ends. While some methodologies have been...
This paper presents an advanced diascopic illumination technique for simultaneously multi-wavelength fluorescence excitation and detection without any filter sets. High-throughput capillary electrophoresis detection can be realized with this simple and novel approach. The developed system is composed of a home-built dark-field condenser for fluorescence excitation and an ultraviolet-visible-near infrared...
The potential environmental and health effects of particles at the nanoscale have not been well studied as of yet. Using silica nanoparticles as a model, this study aims to elucidate some of the uncertainties behind nanoparticle -cellular interactions, in order to determine if the ubiquitous presence of these nanoparticles in our environment could be detrimental to public health and the environment...
A micro-optic-fluidic spectrometer is demonstrated for the fluorescence detection of moving particles in a microfluidic chip. The object is excited within an integrated 3D liquid-liquid waveguide and its fluorescence spectrum is real-time recorded with resolution of 10 nm. Three different types of fluorescence beads were tested and distinguished by this spectrometer which holds a promising new concept...
A new probe for scanning near-field optical microscopy (SNOM) that exploits field concentration at a metal tip allows to image single dye molecules at a resolution far beyond the diffraction limit. This probe combines the high resolution of the apertureless SNOM with the single molecule sensitivity of the aperture SNOM. An image of single Cy-3 fluorophores covalently attached to the termini of DNA...
Direct temperature measurement inside the micro chamber, in the order of few femtoliter in volume, is the key technology for the up coming experiments in single molecular observation. The temperature inside the cylinder shaped micro chamber, 8 mum indiameter and 2.2 mum in height, was directly detected. The temperature was calculated from the temperature dependent fluorescent intensity of Rhodamine...
The laser-induced fluorescence spectroscopy was used to investigate to investigate water stress in orange trees (Citrus aurantium LN). In our experiments, six three were under water stress conditions and four in regular conditions, as control. The fluorescence spectroscopy was investigated by using a 442-nm 15 mW He-Cd gas multimode discharge laser and 532-nm 10 mW Nd3+:YAG laser for fourteen days...
Fluorescence can be tailored by the vicinity of a metallic interface. Modifying the intensity and the spatial distribution of the emitter radiation as well as the energy transfer between donor and acceptor can benefit to biological applications. Fluorescence enhancement has been investigated by one and two-photon fluorescence microscopy with time and spectral resolution. Besides, the spatial emission...
We have investigated a commercial fluorescence immunoassay (FIA) reader (KRYPTOR-Cezanne), designed for homogeneous immunoassays, which is based on a time-resolved measurement of Forster resonance energy transfer (FRET) from a europium cryptate (Eu-TBP) donor to a crosslinked allophycocyanin (XL665) acceptor. The system uses a nitrogen laser as excitation source and a two-channel PMT detection system...
The field of microfluidics has developed rapidly with promising applications such as 'lab on a chip' devices for micro-assays and efficient chemical reactions and processes, including e.g. DNA sequencing. The design of microfluidic devices requires an understanding of fluid flow and dynamics, which we demonstrate can be enhanced by direct observation using (time-resolved) multi-dimensional fluorescence...
We have demonstrated the possibility to obtain the orientation of single CdSe quantum dots by optical defocused microscopy. At the single molecule level, it is a useful tool to analyse the modification of the spontaneous emission of a single emitter close to an interface, it could also be used to study processes such as energy transfer between a quantum dot and a fluorophore. In the field of biological...
The imaging of molecular events in the complex physiological interplay between organelles, cells, tissues, and organs in the whole organism is now more practical through the use of small-animal imaging technologies. Radionuclide based molecular imaging using single photon emission computed tomography (SPECT) and positron emission tomography (PET) scanners have been used for imaging intracellular enzymes,...
New advances in pulse generators for real-time fluorescence microscopy of cell electroperturbation are presented. A MicroPulser described here is constructed as part of a fluorescence imaging system to expose four independent samples on a single microscope slide to nanosecond pulsed electric fields. The all solid-state pulse generator is a totem pole design delivering 450 V pulses with 0.5-ohm source...
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