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The biosynthesis of 5-(3-buten-1-ynyl)-2,2′-bithiophene was studied in root cultures of Tagetes patula. Organ cultures were grown with [U- 13 C 6 ]glucose or [1- 13 C]glucose. The bithiopene and amino acids from cell protein were isolated and analysed by quantitative NMR spectroscopy. Retrobiosynthetic analysis establish acetyl-CoA or a closely related compound (e.g. malonyl-CoA)...
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