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A bacterial strain Pseudomonas sp. Zjwp-14 was screened out from soil, and used for the biotransformation of DL-2-amino-Δ2-thiazoline-4-carbonic acid to L-cysteine. This strain was identified based on its morphological, physiological characterization, 16S rDNA sequence determination and phylogenetic analysis. The bioconversion process was optimized by examining some key factors including cell cultivation...
With genome-wide screening of N. vectensis, two new groups of Polinton were identified, beside five Polinton groups deposited in Repbase. The typical N. vectensis Polinton is in length of 15-20kb and has long TIRs with a 6-bp TSDs. it encodes as many as 6 different proteins, including retroviral-like integrase (INT), adenovirus protease (PRO), DNA polymerase B (POLB) and putative ATPase (ATP). These...
Hepialus is the host of Cordyceps sinensis which is a well-known and valuable Traditional Chinese Medicine. The mitochondrial genes of Cytochrome Oxidase Subunit I (CO I), Cytochrome Oxidase Subunit II (CO II) and Cytochrome b (Cytb) were cloned from Hepialus in partial areas of Qinghai-Tibet Plateau. The sequences were analysed, and the molecular phylogenetic trees were constructed. The results showed...
It is important to develop computational methods to predict target genes of a transcription factor. In this paper, we developed a phylogenetic footprinting method to effectively identify target genes on the whole genome scale. Appling the method to 31 transcription factors, we improved the quality of the initial motifs, and predicted additional target genes that are supported by gene ontology analysis...
With the increase of sequence information, many methods have been used in detecting sequence similarities. In order to avoid the shortcomings of the traditional method based on sequences alignment such as high time and space complexity, component vector method has been successfully used in the reconstruction of phylogenetic tree using genome (proteome) or conserved molecular sequences. With the aim...
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