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A sensitive and specific LC–MS/MS method employing positive electrospray ionization for the determination of ARQ 501 (β-lapachone) in (nu/nu) mouse plasma and tumor tissue is described. Samples were processed using protein precipitation with acetonitrile. A d6 analog of ARQ 501 was used as the internal standard (IS). The analytes were separated using a Zorbax SB8 column (30mm×2.1mm i.d. 5μm particle...
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