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The mechanics of many biological processes can only be uncovered through the analysis of spatio-temporal data. Kymographs are a popular tool for visualising dynamic processes whose movements can be mapped into a single dimension, such as mitosis, or cell division. However, global movements of a cell means the region of interest (ROI) used to create the kymograph must move with each frame. Here we...
Advanced fluorescence live-cell imaging allows visualising the spatio-temporal dynamics of cellular processes in great detail. A fundamental problem is quantifying such processes in cells that continuously change shape and move. Here I outline recent developments and future challenges in three areas, highlighting the need for a very diverse range of methods to tackle seemingly similar problems: I)...
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