The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
This paper reports a developed DNA hybridization detection method using gold-streptavidin conjugates as a reporter, the silver enhancement reaction to magnify the detection signal, and a flatbed scanner to detect the hybridization signal. There was a significant difference in grayscale between the experimental samples and the negative control after 18 min. The detection limit for a DNA sample is 8...
A 10 nm nanogold particle was used to label monoclone CEA antibody to prepare an immunonanogold resonance scattering probe (AuCEAAb) for detection of CEA. In the pH 6.8 Na2HPO4-NaH2PO4 buffer solution and in the presence of PEG-6000, the immunoreaction between CEA and AuCEAAb took place to form the immunonanogold complex that can be removed by centrifugation technique. The supernatant containing AuCEAAb...
We present a broadly-applicable nanodeposition tool, the Nanofountain Probe, for direct-write fabrication of functional nanostructures using liquid molecular ??inks??. Examples of nanopatterning of biomolecules, catalysts for subsequent nanostructure growth, and functional nanoparticle arrays for nanosystems fabrication and single cell studies will be presented. Recent developments in the use of the...
A platform for in situ and real-time measurement of protein induced conformational changes in dsDNA is presented. We demonstrate protein induced bending upon sequence specific binding of integration host factor (IHF) to dsDNA probes.
We combine electrical orientation of surface bound dsDNA probes with an optical technique for kinetic measurement of protein-induced conformational changes in DNA. The effects of probe surface density and protein variation are investigated.
A novel homogeneous ECL biosensing method for the determination of thrombin was designed based on DNA hybridization. As a model system, thrombin-binding aptamer was utilized as molecular recognition element and thrombin as a target analyte and ABEI derivatives as aptamer ECL label. In the absence of thrombin, thrombin-binding aptamer-tagged with ABEI produced a weak ECL signal. In the presence of...
In this work, the effect of gold nanoparticles (AuNPs) with diameters of around 5, 10 and 20 nm on PCR efficiency is evaluated respectively using a real-time PCR machine. Gold nanoparticles show no obvious effect on PCR at low particle concentration. When the concentration is increased, PCR inhibition is observed. At the same particle concentration, gold nanoparticles of different sizes show different...
We present a microfabricated electrochemical probe (EP) designed for the detection of biomolecules released by dissociated cells and which is operated on an inverted microscope with real time imaging. The characteristics of the EP are (i) a geometry with a flat, blunt tip that is 200 mum wide, and (ii) two nanoelectrodes that are 200 nm thick, 30 mum long, and spaced 30 mum from one another. The two...
This paper proposes a novel design of a reusable biosensor for in-situ and continuous protein detection. The sensing probe was practiced by the integration of a fiber optic interferometry (FOI) sensor accommodated in a micro opto-fluidic chip covered with a microdialysis membrane and surface regeneration mechanism for regenerable detection. Gold-nanoparticles (GNPs) labeled anti-rabbit IgG was used...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.