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Although the formation mechanism of biogenically metallic nanoparticles is broadly associated to enzyme mediation, major attention has been given to the role of proteins and peptides in oxido-reduction of metallic ions leading to these nanostructures. Among the wide range of biomolecules that can act not only as capping agents but also as non-enzymatic agents to form nanoparticles, disulphide bridge-containing...
We present microcantilever-based sensors for the characterization of biopolymer degradation by enzymes. Thin films of Poly(L-lactide) (PLLA) were spray-coated onto SU-8 cantilevers with well-known material properties and dimensions. The micromechanical sensors were immersed in solutions of proteinase K to investigate enzymatic degradation of PLLA. A decrease of the resonance frequency after immersion...
Glutathione peroxidase (GPx) and other selenoproteins (SeP) are involved in the detoxification of reactive oxygen species (ROS). ROS is implicated in preeclampsia (PE). Generation of ROS and antioxidant effect of total GPx in women with PE was estimated. Since we found a significant decrease in total GPx level of women with PE, we were motivated to characterize at a molecular level SeP present in...
In this work, the effect of gold nanoparticles (AuNPs) with diameters of around 5, 10 and 20 nm on PCR efficiency is evaluated respectively using a real-time PCR machine. Gold nanoparticles show no obvious effect on PCR at low particle concentration. When the concentration is increased, PCR inhibition is observed. At the same particle concentration, gold nanoparticles of different sizes show different...
Miniaturised point-of-care cardiac marker sensors are being developed, based on impedimetric sensing of cardiac enzyme capture by an antibody layer immobilised on a planar gold electrode sensor. Gold/Ti-on-glass substrates have been used, in a 2 electrode configuration, with antibodies immobilised on the working electrode. Microfluidic structures have been fabricated by a CO2 laser, in 25 mum thick...
Miniaturised point-of-care cardiac marker sensors are being developed, based on impedimetric sensing of cardiac enzyme capture by an antibody layer immobilised on a planar gold electrode sensor. Gold/Ti-on-glass substrates have been used, in a 2 electrode configuration, with antibodies immobilised on the working electrode. Microfluidic structures have been fabricated by a CO2 laser, in 25 mum thick...
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