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The protein adsorption is a crucial but complex phenomenon in many applications, like biosensors, biomaterials. The amount, orientation and biological activity of adsorbed proteins are heavily dependent upon the structure of protein, like widely-applied Bovine Serum Albumin (BSA), and the properties of the substrate. At some extent, to explore the physicochemical properties and dynamics interaction...
In this work, the effect of gold nanoparticles (AuNPs) with diameters of around 5, 10 and 20 nm on PCR efficiency is evaluated respectively using a real-time PCR machine. Gold nanoparticles show no obvious effect on PCR at low particle concentration. When the concentration is increased, PCR inhibition is observed. At the same particle concentration, gold nanoparticles of different sizes show different...
This paper presents an attempt to use two substrate types silicon and glass for microarray technique, in order to find the best chemistry surface for immobilization of proteins in microarray technology. Different chemical substrate were studied (glass slides and silicon slides coated with gold and poly-L-lysine) printed with BSA and marked with CY3. The slides were printed and after that washed with...
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