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This paper reports a step-wise protocol to immobilize corresponding antibody on gold electrodes for electrochemical measurement of Osteocalcin (Oc), a bone turnover marker, levels in human serum. To our knowledge this is the first such sensor developed for Oc measurement. The magnitude of electrochemical response current was linearly dependent on Oc concentration with an R square of 0.99. The detection...
Five different Nano-materials (NMs) were used for making hybrid conjugates with Protein-A (PrA), a biomolecule that binds specifically with antibodies (Abs). The choice of NMs (Gold, CdS, SrTiO3, Graphene Oxide and Polystyrene) exhibit both inorganic and organic NMs, with a wide range of electrical characteristics i.e., from metal, semiconductor to non conducting dielectric materials. The NMs-PrA...
We present a unique microfluidic platform to allow for quick and sensitive probing of protein adsorption to various functionalized surfaces. The ability to tailor a sensor surface for a specific analyte is crucial for the successful application of portable gas and fluid sensors and is of great interest to the drug screening community. However, choosing the correct surface chemistry to either block...
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