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Objective Molecular methods to detect diarrheal pathogens are increasingly being used in place of conventional methods. We compared a new multiplex real-time PCR assay for detection of both bacterial and viral gastroenteritis agents, the Allplex™ Gastrointestinal Panel Assays (AGPA), to conventional methods (stool culture for bacterial pathogens and electron microscopy (EM) for viral pathogens). ...
Acute gastroenteritis presenting with diarrhoea and or vomiting is extremely common in childhood, and, is extremely difficult to differentiate from community acquired infection. Additionally the aetiology of community acquired acute gastroenteritis varies depending upon setting (developed or developing), age, season and testing modality. In all settings, rotavirus remains the most common cause of...
Chrysanthemum plants are subject to serious virus diseases, so detection and identification of virus pathogens is important to prevent the virus spread. A reliable one‐step multiplex RT‐PCR was developed to simultaneously detect two viruses and two viriods: chrysanthemum virus B, tomato Aspermy virus, chrysanthemum stunt viroid and chrysanthemum chlorotic mottle viroid. In addition, we investigated...
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