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The purpose of this study was to determine if nicotine or cotinine inhibits steroidogenesis in the ovarian follicle. Theca interna and granulosa cells were isolated from bovine follicles, cultured with nicotine or cotinine for 24h, and culture media were assayed for androstenedione or estradiol. Treatment of theca interna with 6, 60, and 600μM nicotine decreased (P=<0.002) production of androstenedione...
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