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In vitro brain slice preparations are instrumental in developing our understanding of the nervous system. However, the current lifespan of an acute brain slice is limited to approximately 6–12 hours. This reduces potential experimentation time and leads to considerable waste of neural tissue. We have designed, developed and tested a novel incubation system capable of extending the lifespan of these...
Alcohol abuse leads to cognitive defects, depression, and sleep disturbances. The connection between sleep and circadian disruptions led us to investigate ethanol's effect on the circadian clock. We demonstrated that acute ethanol blocks photic phase shifts in vivo and glutamatergic phase shifts in vitro. Ethanol exposure can lead to acute, rapid and/or chronic tolerance. We determined that the suprachiasmatic...
In vitro networks of neurons typically exhibit bursting behavior. To help the experimenter in characterizing different preparations or identifying the changes possibly induced by specific protocols, we developed a new automated method for detecting both bursts and network bursts from electrophysiological activity recorded by means of micro-electrode arrays. The burst detection algorithm is based on...
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