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The functionality of sulphated polysaccharides is directly related to the degree of sulphation of these polymers. Acid hydrolysis is often carried out to study the kinetics of hydrolysis/chain scission of these polymers and the effect on molecular mass. However, measurement of molecular mass by size-exclusion chromatography (SEC) can be artefactual if the hydrolysis step also leads to desulphation...
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