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The combination of microfluidic systems with laser optical manipulation of suspended objects extends the range of possible investigations in lab-on-chip environments. As an example, mechanical properties of cells can be measured with a specific dual-beam laser trap called the optical stretcher on a single cell basis. The combination of high power laser beams in excess of 1W into a microfluidic environment...
A novel strategy to sort the cells of interest (White Blood Cells (leukocytes)) by selectively lysing the Red Blood Cells (erythrocytes) in a miniaturized microfluidic device is presented. Various methods to lyse cells on a chip exist i.e. electrical, mechanical, chemical and thermal but they need integration of electrodes, traps, reservoirs, heaters, etc which is often difficult at microscale. On...
In this paper, we report a new and simple method of patterning polydimethylsiloxane (PDMS) directly under normal ambient light for rapid prototyping of disposable microfluidic lab-on-a-chips (LOCs). The photodefinable PDMS (photoPDMS) is positive-acting and only sensitive to light below 365 nm, which permits processing outside a gold room. A parametric study was conducted to optimize this novel fabrication...
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