The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
Soluble catechol-O-methyltransferase (S-COMT) is an important target protein due to its involvement in some human disorders. The main scope of the work was to develop an approach to purify and identify S-COMT. Its isoelectric point was 5.0 determined with Offgel. A simplified process including extraction, selective precipitation, ammonium sulfate precipitation, gel filtration chromatography and anion...
Non-mitogenic form of recombinant Human acidic fibroblast growth factor (nrhaFGF) retained the cardio and neuro-protective characters of the wild-type aFGF while decreased the potential side effects like mitogenesis activity. In this paper, nrhaFGF was successfully expressed in Escherichia coli and yielded about 5% of the total soluble protein in the 10L fermentation tank. the succeedent chromatography,...
Nucleotide sequences of the 16S rRNA gene of Thermobifida (formerly Thermomonospora) Q-0 was analyzed by MEGA v.3, and the phylogenetic tree showed the relationship between different strains in Thermobifida genus. A carboxymethyl cellulase I (CMCase I) was purified by fractional ammonium sulphate precipitation and ion exchange chromatography on DEAE-Sephadex. The enzyme showed the highest activity...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.