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Generation of hydroxyl radical (middotOH), one of the major reactive species in the ozonation of water, was directly measured with an electron spin resonance (ESR) spectroscopy. Hydroxyl radical in ozonated water was trapped with 5,5-dimethyl-pyrrolidine-1-oxyl (DMPO) as a stable radical, DMPO-OH, and the amount of DMPO-OH was measured with an ESR spectrometer. The ESR signal of DMPO-OH increased...
Impairment of mitochondrial functions has been found in ethanol-induced liver injury. Ethanol can be oxidized to the 1-hydroxyethyl radical (HER) by rat liver microsomal systems. Experiments were carried out to evaluate the ability of HER to cause mitochondrial swelling as an indicator of the mitochondrial permeability transition (MPT). Electron spin resonance (ESR) spectroscopy was used to detect...
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