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Human cytotoxic T-lymphocyte antigen 4 (hCTLA4), an immunosuppressive agent, was expressed as a fusion protein to produce a multimeric form. The fusion partner was Hepatitis B surface antigen (HBsAg), which was fused to the N-terminal extracellular domain of hCTLA4. The expression vector, pPIC3.5hc, constructed for the insertion of the fusion gene into the pPIC3.5 vector with the AOX promoter, was...
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