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Hydroponic isotope labelling of entire plants (HILEP) is a cost-effective method enabling metabolic labelling of whole and mature plants with a stable isotope such as 15 N. By utilising hydroponic media that contain 15 N inorganic salts as the sole nitrogen source, near to 100% 15 N-labelling of proteins can be achieved. In this study, it is shown that HILEP, in combination...
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