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Vascularization for sufficient supplies of oxygen/nutrients and removal of waste in thicker tissue is one of the major challenges in tissue engineering. Here, we used three-dimensional bio-printing technology to engineer a vascular structure within hydrogel scaffold. Through layer-by-layer approach, we seeded endothelial cells in tubular form, which is embedded within three-dimensional collagen scaffold...
Early perfusion is essential for survival and function of engineered tissues. A promising solution to this problem is the development of stable microvessels within an engineered construct. Transplantation of human endothelial cells within protein gels has been shown to form functional vascular networks both in vitro and after implantation into immunodeficient mice. Co-transplantation of human pericytes,...
Cells cultured within a 3D environment acquire phenotypes and respond to stimuli analogous to in vivo development. This approach can be applied to the study of tumorigenesis in vitro. In this study, collagen I hydrogels were engineered as a platform for in vitro solid tumor development. Cell seeding density, scaffold thickness, and matrix stiffness were varied to characterize the development of a...
A peptide hydrogel for in vitro culture of chondrocytes was prepared and investigated based on the designed and synthesised peptide SGGAGGAGGAGGAGGS. Chondrocytes cultured in the peptide hydrogels were observed for changes of morphology characteristic, and the chondrocyte-seeded peptide hydrogels at the designed time point were obtained and the analysis of DNA content, histological staining and immuno-histochemistry...
In this study, we demonstrate the formation of 3D cell-adhesive hydrogels exhibiting well-defined spatial variation in stiffness using our previously developed microfluidics-based lithography technique. PEG monoacrylate-linked bovine fibrinogen (PEG-fibrinogen) is photopolymerized into specific, user-defined shapes inside a microchannel, and successive cycles of fabrication result in a heterogeneous...
Vascularization for adequate supplies of oxygen and nutrients is one of the challenges in tissue engineering. We report the three-dimensional cell printing technology to construct vascular structure in hydrogel scaffold which consist of endothelial cells and collagen scaffold. We constructed multi-layered, inter-connected channels within collagen hydrogel through layer-by-layer approach. Collagen...
Tissues formed by cells seeded in hydrogels are used for biotechnology, cell-based assays, and tissue engineering. We have previously presented a cell-micropatterning technique that localizes live cells within hydrogels using dielectrophoretic (DEP) forces, and have demonstrated the ability to modulate tissue function through the control of microscale cell architecture. In this study, we developed...
In this study, to improve efficiency and functionality of cell-based biosensor microarrays, we introduced nano-topographical features on cell adhesive region. Micropatterned surface was created by poly (ethylene glycol) (PEG) hydrogel microstructures via photolithography. Nano-topographical features were introduced using polymeric nanofibers which were produced by electrospinning technique. Combining...
Cell-based biosensors (CBBs) are becoming an important tool for biosecurity applications and rapid diagnostics. For current CBBs technology, cell immobilization and high throughput fabrication are the main challenges. To address these in this study, the feasibility of bioprinting cell-laden hydrogel to fabricate CBBs at high throughput was investigated and cell response was tracked by using lensless...
Bone exhibits hierarchical levels of organization from macroscopic to microscopic, and nano- length scales. Furthermore, multiple bioactive peptides, as part of the collagenous and non-collagenous water soluble glycoproteins and proteoglycans in the bone ECM, interact with progenitor BMS cells to initiate the cascade of chemotaxis, differentiation, and mineralization. In this work, a nanofiber hydrogel/apatite...
In this study, we report a newly developed three-dimensional (3D) biological printer using non-contact, electromechanical microvalves with a nozzle diameter of 150 mum. To control and utilize this printer for life science applications, we developed an easy-to-use control software with a graphic user interface (GUI). First, using the printer, we tested the viability of dispensed mammalian cells after...
Today, one of the limitations associated with cartilage grafting is the lack of bonding between the implanted materials and the natural tissue. Helical rosette nanotubes (HRNs) are novel biomimetic self-assembled supramolecular structures whose basic building blocks are DNA base-pairs, which can solidify into a viscous gel at body temperatures in short periods of time. HRNs are similar in size to...
We are interested in engineering cartilage that is resistant to arthritic disease. We hypothesized that suppression of terminal differentiation pathways would lead to decreased chondrocyte catabolic response to inflammatory cytokines and used a Bcl-2 over-expression gene therapy approach targeting chondrocyte apoptosis. Retrovirally transduced chondrocytes were cultured in 1.25% alginate hydrogels...
We created 3D tissue constructs epitaxially by printing cell-laden hydrogel droplets. The ability to bioengineer 3D tissues is a powerful new approach to treat diverse diseases such as cancer, loss of tissue function, or organ failure. Inspired by the molecular beam epitaxy technique, a common semiconductor manufacturing technology, we present a platform that prints the first example of a 3D smooth...
Human embryonic germ (hEG) cells have the potential to self-renew over long periods of time and differentiate into various lineages. Cells derived from embryoid bodies of hEG cells express a broad spectrum of gene markers and have been induced towards cells of ecto-dermal and recently endo-dermal and mesenchymal lineages. LVEC cells express a number of surface marker proteins characteristic of mesenchymal...
Human embryonic germ (hEG) cells have the potential to self-renew over long periods of time and differentiate into various lineages. Cells derived from embryoid bodies of hEG cells express a broad spectrum of gene markers and have been induced towards cells of ecto-dermal and recently endo-dermal and mesenchymal lineages. LVEC cells express a number of surface marker proteins characteristic of mesenchymal...
Hydrogels based on 2-hydroxyethyl methacrylate (HEMA) crosslinked with tetraethylene glycol (TEGDA) and molecularly engineered using two methacrylate-based monomers, poly (ethylene glycol) (200) monomethacrylate (PEGMA) and 2-methacryloyloxyethyl phosphorylcholine (MPC) in the range of 0.0 - 0.5 mol % and 0-10 mol % respectively were investigated. Hydration studies demonstrated up to a 93.8% increase...
The biocompatibility of biosmart polymer membranes synthesized from cross-linkable (2-hydroxyethyl methacrylate) (HEMA) and tetraethylene glycol diacrylate and containing different mole-percent polyethylene glycol methacrylate (PEGMA) and methacryloyloxyethyl phosphorylcholine (MPC), a phosphorylcholine-containing co-monomer, was investigated. The cytotoxicity (cell viability and proliferation) and...
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