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We report here an in-depth study of the permeability of ultrathin parylene-C (0.15∓0.80 μm thick) by measuring the diffusion coefficients of FITC-dextran molecules with different molecular weights (MW). As a validation, we have successfully demonstrated the application of the ultrathin semipermeable membranes in perfusion cancer cell culture. The results show that, with a combined biocompatibility...
We report integration of CdTe quantum dot (QD) film with LnCap (prostate cancer) cell and CdSe QD probes with cortical neurons for control of cellular activity. We demonstrate the remote switching of cellular activity by exciting QDs with light. Changes in membrane potential and ionic currents are recorded using the patch-clamp method. Upon excitation, the cell shows activation of ion channels and...
The U.S. FDA allows a maximum of 72 mg of caffeine per 12 oz. serving (6 mg/oz) and the tolerance to sleep disruption effects of caffeine develops after consuming 400 mg/day of caffeine 3 times for a week. However, it is still hard for people to estimate how much caffeine is intake daily. On the other hand, (-)epigallocatechin gallate (EGCG) is studied a potent antioxidant that may have therapeutic...
This paper presents a method of capturing viable circulating tumor cells (CTC) from human whole blood using constant-pressure-driven filtration through a specially designed parylene-C membrane "slot" filter. More than 90% viable cancer cells could be recovered from whole blood using the slot filter, with minimal non-cancer blood cells left on the filter. The feasibility of the telomerase...
Submicrosecond megavolt-per-meter pulsed electric fields drastically affect intracellular membranes and induce apoptosis. For this reason the fields are recently used as a purely electrical cancer therapy that kills tumors without hyperthermia or drugs. But as far as we know the operational characteristic of this method (amplitude-duration relationship) has never been calculated theoretically. We...
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