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This paper reports a low-cost fabrication method for mass production of free standing cyclic olefin copolymer (COC) membranes embedded with micro pore arrays. We show that the method creates suspended membranes with thickness of 2μm and micro pores having diameter ranging from 3–5μm. We integrated the membrane in a microfluidic system designed to mimic the endothelial barrier in bone marrow sinusoids...
This report describes preliminary results of reconstitution of an in vivo-like multi-layered placental barrier without porous membrane support. We used thin collagen membrane (10 μm in thickness, commercially available) as permeable support of placental barrier structure and cultured BeWo trophoblastic cells and HUVECs on either side of the membrane. Co-cultured cells deposited abundant basement membrane...
In this paper, we describe the localized and selective electrical stimulation of single cells using a three-dimensional electrode array. The chip consisted of 84 nail-like electrodes with a stimulation surface of 0.8 um and interelectrode distances as small as 3 um. N2A cells were used to compare bipolar stimulation between one electrode in- and one outside the cell on the one hand, and two electrodes...
Electrical stimulation was applied to drive rat skeletal muscle cells. Then this driving force was utilized to fabricate a diaphragm type micropump. First, a skeletal muscle cell sheet, which consisted of skeletal muscle cells on a PDMS sheet, was fabricated to extract the drive force from the cells. These cells are known to align along a micro pattern. We took advantage of this principle and patterned...
The fabrication and characterization of a micro- chamber electrode array for electrical and electrochemical studies of individual biological cells are presented. The geometry was tailored specifically for measurements from sensory hair cells isolated from the cochlea of the mammalian inner ear. Conventional micro- electromechanical system (MEMS) fabrication techniques were combined with a heat-sealing...
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