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While there is evidence of nitric oxide (NO)-dependent signalling via the second messenger cyclic guanosine 3′,5′-monophosphate (cGMP) in plants, guanylate cyclases (GCs), enzymes that catalyse the formation of cGMP from guanosine 5′-triphosphate (GTP) have until recently remained elusive and none of the candidates identified to-date are NO-dependent. Using both a GC and heme-binding domain specific...
A nitric oxide (NO) electrochemical sensor for hypoxic, tumor cell culture applications is developed. Qualitative analysis of the electrode has been studied by cyclic voltammetry. NO amperometric measurement methods have been extensively analyzed. Square wave voltammetry method is found to be one of the optimal methods for the measurement of NO in cell cultures. A hypoxic tumor cell culture measurement...
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