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Applications of nanotechnology and nano-science have ever-expanding breakthroughs in medicine, agriculture and industries in recent years; therefore, synthesis of metals nanoparticle (NP) has special significance. Synthesis of NPs by chemical methods are long, costly and hazardous for environment so biosynthesis has been developing interest for researchers. In this regard, the extracellular biosynthesis...
The strands of chromosomes are supposed to be split into genic and intergenic regions. Gene identification is an optimization problem in Eukaryotic DNA splicing. An optimal solution is essential which may help in better protein translation. We have proposed a novel approach for gene identification by employing discrete wavelet transforms for noise reduction in DNA sequences and introducing a new indicator...
The partial sequence of the psbA gene from Cladosiphon okamuranus was amplified by PCR and their sequences were analysed. Neighbor-joining method was used to build phylogenetic trees according to the psbA sequence among 11 species, C. okamuranus included, that are affiliated to the algal order Ectocarpales. The results showed that the nucleotide sequence is 737 bp and the sequence shares high homology...
We determined the nucleotide sequence of a portion of the duck enteritis virus (DEV) strain CHv which was suspected to be the UL10 gene(GenBank accession no EU195100.), encoding glycoprotein M(gM). The UL10 gene was cloned and sequenced, which was composed of 1230 nucleotides encoding 409 amino acids, with a predicted molecular mass of 45.752 kDa. We calculated the codon usage bias in the newly identified...
The glycoprotein I (gI) gene homologue of duck plague virus (DPV) was cloned by degenerate polymerase chain reaction (PCR) and sequenced. DPV gI gene open reading frame (ORF) was 1116 bp in length and its primary translation product was a polypeptide of 371 amino acids long. Comparison with other herpesvirus revealed higher similarity, had a close evolution relationship with members of the genus Mardivirus...
β-ketoacyl-ACP synthetase II (KAS II) which catalyzes the elongation of 16:0-ACP to 18:0-ACP, determines the ratio of palmitic acid (16:0) to stearic acid (18:0). Using Rapid Amplification of cDNA Ends (RACE) and a peanut cDNA library we had previously constructed, we isolated a 1867bp cDNA of the KAS II gene containing a 1212bp complete open reading fragment (ORF). Sequence analysis revealed that...
cDNA containing the 3' part sequences of seven Lily symptomless virus (LSV) isolates were obtained by RT-PCR and sequenced. The sequenced region is corresponding to partial CP gene and the entire sequences of 16kDa gene located at the 3' end of LSV genome. The sequence homologies of partial CP and 16 kDa protein genes, compared with other reported isolates, were 87.4% to 100% and 97.9% to 100% respectively...
Eukaryotic transcriptional regulation is complex. Typically, it takes place by cooperative regulation. A number of studies have characterized this aspect in the upstream promoter sequences of Saccharomyces cerevisiae genes. In this paper, we performed an analysis of co-occurrence of motifs in introns. Here, we first conducted a Match™ program to detect single motifs for transcriptional factors. Second,...
With the increase of sequence information, many methods have been used in detecting sequence similarities. In order to avoid the shortcomings of the traditional method based on sequences alignment such as high time and space complexity, component vector method has been successfully used in the reconstruction of phylogenetic tree using genome (proteome) or conserved molecular sequences. With the aim...
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