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Alignment analysis indicated that the nucleotide sequences of Liver X receptors α (LXRα) of Landes goose are highly conserved to other species including chicken, human, rat, mouse and cattle, and the homologies are 95.2%, 76.3%, 74.5%, 74.3% and 74.9%, respectively. Furthermore, molecular phylogeny analyses have shown that LXRα were divided into two main evolution branches, one was the mammals branch,...
SET and MYND domain-containing protein 3 (SMYD3) is a novel histone methyltransferase involved in human carcinogenesis. To establish the transgenic cell model of SMYD3 for the further studies on the characteristics and functions of SMYD3, in the present study, the human SMYD3 gene was subcloned into pIRES2-EGFP eukaryotic expressing vector. The recombinant plasmid was transfected into NIH3T3 cells...
In order to study the apoptosis-induction effects of exponential decay nanosecond pulsed electric fields (EDnsPEF) in vivo, tumor models in 20 female BALB/c nude mice were established by inoculating them with human melanoma cells A375. These mice were randomly divided into treated group (exposed to EDnsPEF with intensity of 20 kV/cm and duration of 300 ns) and control group equally. Twenty days later,...
MicroRNAs (miRNAs) have been found in diverse organisms and play critical role in gene expression regulations of many essential cellular processes. Discovery of miRNAs and identification of their target genes are fundamental to the study of such regulatory circuits. To distinguish the real pre-miRNA from other stem loop hairpins with similar stem loop (pseudo pre-miRNA) is an important task in molecular...
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