The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
MicroRNAs (miRNAs) are small noncoding RNAs which regulate gene expression. In this research, we used the Multiz alignments data of 44 Vertebrates to retrieve syntenic homolog sequences. And we identified 37 members of miR-1302 family among 71 potential homolog sequences. Then we analyzed the 37 homologs of hsa-mir-1302s by RepeatMasker and found all of these sequences are derived from MER53 elements...
Propose: Idiopathic orbital inflammatory pseudotumors (IOIP) are inflammatory pseudotumors (IPT) of unknown etiology that develop in the orbit. Due to the lack of well-defined pathogenic mechanisms and diagnostic markers, diagnosis and treatment of this disease remain a significant challenge. Therefore, the aims of this study are to define the etiological factors of IOIP and to identify potential...
Bidirectional gene pairs have received considerable attention because this unique genomic organization is so prevalent in vertebrate genome that it covers more than 10% of the whole genes in human. To study the character of these adjacent head-to-head gene pairs and to improve the understanding of the difference between bi- and unidirectional genes, we identified all the bidirectional gene pair members...
Endoplasmic reticulum stress(ERS)is a kind of sub-cellular pathological process which result from calcium disorderliness and protein improper folding. And endoplasmic reticulum stress induced apoptosis is a novel pathway which different from the mitochondria-initiated intrinsic pathway and the death receptor-triggered extrinsic pathway. Trichosanthin (TCS), a type I ribosome-inactivating protein,...
Alignment analysis indicated that the nucleotide sequences of Liver X receptors α (LXRα) of Landes goose are highly conserved to other species including chicken, human, rat, mouse and cattle, and the homologies are 95.2%, 76.3%, 74.5%, 74.3% and 74.9%, respectively. Furthermore, molecular phylogeny analyses have shown that LXRα were divided into two main evolution branches, one was the mammals branch,...
MicroRNAs (miRNAs) are single-stranded noncoding small RNAs, frequently expressed as clusters. The co-expressed miRNA clusters are pivotal in coordinately regulating multiple processes, including embryonic development, cell cycles and cell differentiation. In the study, relative expression analysis of miRNA cluster was performed based on isomiRs from high throughput DNA sequencing data using SOLiD...
SET and MYND domain-containing protein 3 (SMYD3) is a novel histone methyltransferase involved in human carcinogenesis. To establish the transgenic cell model of SMYD3 for the further studies on the characteristics and functions of SMYD3, in the present study, the human SMYD3 gene was subcloned into pIRES2-EGFP eukaryotic expressing vector. The recombinant plasmid was transfected into NIH3T3 cells...
Purpose: The etiology of idiopathic orbital inflammatory pseudotumors (IOIP) is unknown. Epstein-Barr virus (EBV) infection, which causes significant lymphocyte infiltration, has been proposed to be involved in IOIP. This study tries to validate the relationship between EBV infection and the development of IOIP. Methods: Eleven surgical samples from IOIP patients and four non-IOIP control samples...
This study was comparison of MGMT and ERBB2 expression in human gliomas. RT-PCR method was used to determine the mRNA levels of MGMT and ErbB2 gene in 42 clinical glioma specimens,There was significant concordance between the expression of MGMT and ErbB2 (p < 0.01). MGMT and ErbB are highly remarkably related each other. The results suggest that there may be in relationship among MGMT and ErbB2...
To investigate the effect of shRNA-SR-PSOX (Scavenger receptor binds phosphatidylserine and oxidized lipoprotein) on smooth muscle cells (SMC) migration, we designed and synthesised the shRNA oligonucleotide targeted human SR-PSOX gene, and constructed the shRNA lentiviral expression system in vitro to infect HepG2 cell line, and the silencing efficiency was assayed by real-time reverse transcription-polymerase...
Alternative splicing is the RNA splicing variation mechanism. The exon isoforms lead to numerous different expressions through the alternative splicing. It's imperative to find the carcinogenic gene from the massive quantity of genes and understand the expressed isoforms. In this paper, the statistical procedures are applied on the colon cancer sample data of Affymetrix GeneChip Human Exon 1.0 ST...
Here we demonstrate the ability to perform differential gene expression experiments using messenger RNA (mRNA) isolated from crude cell lysates using a plastic microfluidic solid phase extraction column. The microfluidic columns (100 mum by 100 mum by 1.5 cm) were fabricated in a cyclic polyolefin by hot-embossing with an electroformed master-mold. The solid-phase consisted of a photopolymerized microporous...
To investigate the effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD) on DNA damage and expression of DNA damage-inducible genes in human hepatoma HapG2 cells. Cell survival was measured using MTT metabolic viability assay. Single cell gel assay was applied to determine the DNA damage .The level of gene expression was measured by reverse transcription-polymerase chain reaction . The cell survival...
The background of this study is Tanshinone was thought to be a potential antitumor drug. In this study, we investigated the apoptosis of hepatoma HepG2 induced by Tanshinone II-A as well as the involved molecules. The inhibitory effects on cell proliferation of Tanshinone II-A were assessed by MTT method. DNA fragmentation was evaluated by agarose gel electrophoresis. Cell cycle and apoptotic rates...
Epidermal stem cells have the characteristic of the undifferentiated cells in the morphology, and are the biological source of the skin and its subsidiary in the development, repair and reconstruction. The proliferation and differentiation of epidermal stem cell are influenced by many factors, such as integrin, signal transduction pathway and cytokines. Current researches have not yet been able to...
This Small nucleolar RNA (snoRNA) are a group of non-protein-coding RNA molecules among hundreds of others in the human genome. These molecules bind specifically to other cellular RNA targets via base pairing to form short, double-stranded structures. This binding causes the snoRNA targets to undergo specific chemical modifications. There are a number of (orphan) snoRNAs whose targets are still unknown;...
In this paper we report an ongoing project for identifying human cytomegalovirus (HCMV) micro RNAs (miRNA) expressed in infected human cells using the new massive parallel sequencing technology with the Solexa Sequencer. We developed a data processing pipeline for analyzing such data including mapping segments to genomes, detecting highly expressed sequences and their loci, comparing sequences to...
Coding and non-coding gene prediction is still a challenge. Diverse computer-based tools have been created to screen sequences using elaborate strategies for gene prediction. Many of these implement various statistical tests to measure the plausibility of the prediction but until now, a comprehensive negative control did not exist. We developed an algorithm that generates sequences with characteristics...
For almost three decades, cancer was thought to result from changes in the structure and/or expression of protein coding genes. The discovery of thousands of genes that produce noncoding RNA (ncRNA) transcripts in the past few years suggested that the molecular biology of cancer is much more complex. MicroRNAs (miRNAs), an important group of ncRNAs, have recently been associated with tumorigenesis...
RNA interference (RNAi) is mediated by 21 nucleotides of short interfering RNA (siRNA) through sequence-specific cleavage of the cognate transcript. The position-dependent function of ribonucleotide residues in siRNA was analyzed by systematic DNA substitution. The results indicated that eight nucleotides from the 5' end of the guide strand and its complementary sequence are replaceable with DNA counterparts...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.