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Using a double-label immunofluorescence method, we analyzed the time course of the appearance of Fos and Jun in the nuclei of supraoptic nucleus (SON) neurons following intraperitoneal injection of hypertonic saline. Fos and Jun immunostaining appeared within 30 min, peaked at 90-120 min, and disappeared 4-5 h later. At all time points (30, 60, 120, 180, 240 min postinjection), colocalized Fos and...
Gamma-aminobutyric acid (GABA) is known to inhibit the electrical and secretory activity of oxytocin and vasopressin neurones located in the supraoptic and paraventricular nuclei following osmotic, cardiovascular or suckling stimuli. To understand fully the nature of GABA actions on these magnocellular neurones it is important to define the heteropentameric GABA A receptor proteins they express...
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