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The aim of this study was to detect Mycoplasma species in the respiratory tract of 110, 310 and 510 day-old groups of cattle by serological, bacteriological and histopathological investigations. Antibodies against M. bovis were found in 75% of the 110 day-old, in 50% – of the 310 day-old and in 55% – of the 510 day-old groups of cattle. Bacteriological examination of the samples from nasal cavities...
The aim of this study was to determine the diagnostic specificity, sensitivity and repeatability of four commercially available ELISA kits for the detection of antibodies against bluetongue (BT) virus. The relative specificity of ELISAs was estimated using a panel of sera originated from healthy cattle never vaccinated nor exposed to BT virus. All ELISA kits had a high relative specificity (99.2-99...
A serological study of BoHV-1 distribution was conducted in Lithuania from 2005 to 2009. Antibody level was measured using a commercial ELISA. For serological examination, 15,368 random blood samples from cattle of different age, gender, and size of herd, which was unvaccinated against IBR, were collected in 37 districts. It was registered that 11.97% of BoHV-1 were seropositive samples. It was also...
The aim of this study was to evaluate the occurrence of antibodies to foot-and-mouth disease virus (FMDV) in sera of cattle in Poland. The examinations were performed using the virus neutralization (VN) test and ELISA methods: liquid-phase blocking ELISA (LPBE) and 3ABC-ELISA. During 1993-2001, about 681 000 samples of sera collected from animals held on the territory of Poland were tested. Of about...
The aim of these investigations was to prepare the antigen and control sera for the ELISA. Positive control sera were obtained from 163 cows showing clinical symptoms of H. bovis infection and negative control sera came from 60 cows free from the infection. The antigen for the ELISA was prepared from H. bovis L1 larvae. Three basic fractions were isolated from whole excretory/secretory antigen by...
In a previous paper an ELISA for detecting anti-Brucella antibodies in cattle sera was elaborated and evaluated. The aim of this study was to evaluate the ELISA for testing individual milk samples from cattle. Validation was performed by comparing the results of this test with those in the Rose Bengal Test (RBT), tube agglutination test (TAT), complement fixation test (CFT), Coombs anti-globulin test...
The prevalence of bovine viral diarrhea (BVD) virus infection on the basis of serological testing of cattle has been evaluated. Blood samples were analyzed in micro-seroneutralization test for the presence of BVD virus neutralizing antibodies against 1000 TCID₅₀ of NADL reference strain. Neutralizing antibodies were found in 83 per cent of animals tested. The percentage of seropositive animals was...
An affinity purified Babesia bigemina piroplasm antigen was successfully employed in a Dot-ELISA for detection of antibodies in buffaloes and catle. A low degree of cross-reactivity was seen against putative B. bigemina (Mexican) and B. bovis undiluted reference serum and no cross-reactivity was observed in seru dilutions of 1:10 and above. Theileria annulata did not show any reactivity with B. bigemina...
A study on the seroprevalence of FMD antibodies in cattle in the „ring” vaccination zone was performed. Out of 550 samples of sera examined by LPB ELISA, 240 samples (44%) have been found positive to FMDV type A,O and/or C (ELISA titres > log 1.6). The highest Ievel of antibodies was found for FMDV type O₁BFS; about 10% of tested sera had titres > log 3.01. A half-life of maternal antibodies...
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