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Objective: Venodilatation is a feature of endotoxaemia and sepsis. We have tested directly the hypothesis that three cytokines (IL-1β, TNFα and IL-6) generated during endotoxaemia affect venous tone in humans in vivo by increasing NO generation and explored whether the NO comes from the iNOS or eNOS isoform. Design and intervention: Cytokines were given into a superficial vein in very low doses...
Objective: We have attempted to demonstrate the induction of inducible nitric oxide synthase in human vascular tissue and define the capacity of different cytokines to induce this enzyme. Methods: Segments of human arteries were stimulated with lipopolysaccharide (10 μg/ml), interleukin-1β (5 U/ml), tumor necrosis factor-α (10 U/ml), and interferon-γ (200 U/ml). Cytokines were either used alone or...
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