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A 16 h exposure of U937 cells to 2.5 µM arsenite promotes superoxide (
) formation and inhibition of the activity of aconitase, a
sensitive enzyme. Both responses were abolished by the complex I inhibitor rotenone, or by the respiration‐deficient phenotype. Interestingly, a similar suppressive effect was mediated by a short term pre‐exposure to a low concentration of l‐ascorbic acid (AA),...
Arsenite directly triggers cytochrome c and Smac/DIABLO release in mitochondria isolated from U937 cells. These effects were not observed in mitochondria pre‐exposed for 15 min to 10 µM L‐ascorbic acid (AA). In other experiments, intact cells treated for 24–72 h with arsenite were found to die by apoptosis through a mechanism involving mitochondrial permeability transition. Pre‐exposure (15 min) to...
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