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Chemical modification is a useful method to change the properties of enzymes. Laccase is a phenol oxidase belonging to a multicopper protein, which catalyzes the oxidation of many phenolics. DeniLite IIS, a commercial laccase preparation from the Novozymes China Company, was purified by ammonium sulfate fractional precipitation. Succinic anhydride (SA) was used as a modifier for the chemical modification...
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