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This paper presents the experiments on the breakdown behavior of plant protoplasts under electrical pulses. A microfabricated device was utilized for facilitating single-cell observation and for patterning the electric field distribution that enhanced the efficiency of membrane charging. The objectives of the work are to study the effects of pulse parameters on the breakdown behavior and to investigate...
Electroporation represents one of the techniques used for cell research. By membrane permeabilization, biological samples can be introduced into the cells or intercellular content is released for further analysis providing information for diagnosis and treatment. This techniques is achieved in microfluidic systems with microelectrodes that induce a high electric field in the vicinity of cells to generate...
This paper reports an insulator-based dielectrophoresis (iDEP)-assisted single-cell electroporation chip that can locally electroporate cells with an operation voltage of 1V. The electroporation chip consists of two metal electrodes and a sandwiched dielectric polymer layer with microwells. The dielectric microwell arrays create non-uniform electric fields to generate positive DEP forces to capture...
Advances in microfabrication have introduced new possibilities for automated, high-throughput biomedical investigations and analyses. Physical effects such as dielectrophoresis and electroporation can be used to manipulate particles in solution to coordinate a sequence of bioanalytical processing steps. Dielectrophoresis is accomplished with non-uniform electric fields that can polarize particles...
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