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In this paper, we report a novel quantitative assay for chitinolytic activity of lysozyme. Our assay is based on measuring the reducing sugar ends (RSE) released from the water-soluble macromolecular substrate, half-deacetylated chitosan. RSE reacts with 3-methyl-2-benzothiazolinonehydrazone to produce the chromogenic product that has absorption maxima at 615 and 655nm. We found that measurement of...
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