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A sensitive procedure for the quantification of total protein bovine serum albumen (BSA) in human serum was presented with sequential injection sampling and fluorometric detection. A few microliters of sample and fluorescamine solutions were aspirated into the holding coil to facilitate the reaction of protein with fluorescamine by giving rise to a blue-green–fluorescent derivative. The derivative...
A simple, rapid, sensitive and reproducible fluorometric method for determining microamount protein is developed. The method is based on the reaction between protein and Indigo Carmine (IC) in an aqueous solution, which results in a long-wave fluorescence emission (λ ex =690 nm, λ em =780 nm). The calibration curves of several proteins are given. Under the optimized experimental...
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