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The authors describe a new method for the selective detection of aflatoxin B1 (AFB1) by an off-on signaling procedure in a fluorescence resonance energy transfer (FRET)-based nanobioprobe. An amino-modified aptamer against AFB1 was conjugated to fluorescent polymer dots, containing poly[(9,9-dioctylfluorenyl-2,7-diyl)-co-(1,4-benzo-thiadiazole)] as the fluorophore. Complementary DNA (cDNA) was conjugated...
We report observation of high quantum yield, long-lifetime fluorescence from a red dye BO-PRO-3 excited by resonance energy transfer (RET). The acceptor fluorescence was highly enhanced upon binding to the donor-labeled DNA. A ruthenium complex (Ru) was chosen as a donor in this system because of its long fluorescence lifetime. Both donor and acceptor were non-covalently bound to DNA. Emission from...
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