The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
Benzimidazole derivatives are wide interest because of their biological activity and clinical uses. Short and simple method was developed to control the "carry over impurity" in 5-methhoxy-2-mercaptobenzimidazole. This benzimidazole derivative was used in various drug substance syntheses. The proposed method was short (20 min run time) and validated as per the standard validation guidelines...
Xanthone is one of the major bioactive secondary metabolites in Garcinia mangostana. A simple reversed-phase high performance liquid (RP-HPLC) which equipped with photodiode array detector has been developed to analyze xanthone in rat plasma. The rat plasma was treated with deproteining agent (DA) consists of acetonitrile: propanol (1:1), in the ratio of 2:1 (DA: samples). The mobile phase comprises...
Levodopa (l-dopa), the metabolic precursor of dopamine, has primarily been used for the treatment of Parkinson's disease (PD). A reversed phase high performance liquid chromatography (RP-HPLC) method with UV-detection at room temperature has been developed for analysis of levodopa. The method entailed direct injection of rat plasma sample after deproteinization using acetonitrile — propanol (1:1)...
This research paper reports a novel non-invasive electrochemical technique that is used to elucidate the function of the pancreas. The pathology of the pancreas is not easy to elucidate due to its anatomical location. Various invasive tests are done such as Endoscopic Ultrasound and Endoscopic retrograde cholangiopancreatography (ERCP) to determine its function but they have side effect and are not...
Triterpene acids (TA) from fruiting body of Ganoderma lucidum were prepared by solvent extract method and its components were analyzed by TLC, UV, IR and HPLC. The effects of the extract on cell proliferation were investigated by MTT assay. In vitro experiments showed IC50 values of TA towards BEL 7402 at a low dose of 15.62µg·ml−1 inhibited BEL 7402 very significantly (p<0.01). TA-3 isolated...
How to screen active components of herbal drugs is still a hot topic of pharmacologists and pharmaceutical chemists. The main aim of the study was to establish a strategy to tract the active components in early stage based on the fact, that a compound (component) can not take systematic effect without binding to a biomolecule of organism. Affinity constants (KD or KD) and relative affinity indexes...
Drug metabolism is an important aspect for the study of toxic effect in human being. However, the occurrence of several regulatory issues and high cost involved in experimental study on drug metabolism, restrict the study using human as a model system. Keeping this in view, in the present study, the fungus Aspergillus niser was chosen as a model organism for the study of Clemastine metabolism as it...
To explore how to calculate the effect of solanine on the Michaelis constant and the maximum reaction rate of NAT, high performance liquid chromatography (HPLC) was used, with 2-AF as substrate, and the rate at which 2-AF is acetylated into 2-AAF in intact HepG2 cells or in the cytoplasm of HepG2 cells as the reaction rate. The double reciprocal plot was made, with 1/S (reciprocal of the concentration...
HPLC was used to separate chiral compounds with chiral column. Another method was used in HPLC with chiral mobile phase additives to separate chiral compounds. Using chiral mobile phase additives is a good way to separate the chiral compounds. The structure of the derivative takes an important role in separation. We derivatized 2-heptamine with benzene sulfonyl chloride and dansyl chloride, Using...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.