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Previously, we reported that in vitro exposure of murine embryos to 0.1 μg/ml o,p'-DDT (an estrogenic pesticide) significantly reduced development to blastocyst and mean cell number per embryo, and increased percent cell death by 96 h of culture. The objective of the present study was to determine if developmental injury induced by o,p'-DDT resulted from estrogenic, antiestrogenic, or unrelated adverse...
Culturing pronuclear embryos from CD-1 mice with o,p′-DDT and p,p′-DDT was examined as a means for directly evaluating toxicant risk and for increasing the speed of screening developmental toxicants. Pronuclear (2PN) embryos from CD-1 mice were cultured 96 h in modified Earle’s balanced salt solution containing 0.1% (v/v) ethanol (control) or 10-fold dilutions of 17β-estradiol, o,p′-DDT, or p,p′-DDT...
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