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In this paper, we demonstrate the effectiveness of using statistical shape priors to recover shape descriptors from occluded objects in a level set based variational framework. Parameters that balance curve evolution forces are estimated systematically through embedded discrete Conditional Random Field (CRF). In addition, our approach exploits the benefit of using spectral data to construct a local...
Time-lapse microscopy imaging serves as an important method to measure the cycle progression of individual cells in a large population. The lacking of automatic systems that can deal with large volume of time-lapse optical microscopy imaging is a bottleneck of modern bio-imaging research. In this paper we present an effective computerized method for overlapping cell nuclei detection in time-lapse...
Breathing in asbestos fibers can lead to a number of diseases, the fibers become trapped in the lung and cannot be removed by either coughing or the person's immune system. Atmospheric concentrations of carcinogenic asbestos fibers, have traditionally been measured visually using phase contrast microscopy. However, because this measurement method requires great skill, and has poor reproducibility...
We present a high performance variant of the popular geodesic active contours which are used for splitting cell clusters in microscopy images. Previously, we implemented a linear pipelined version that incorporates as many cues as possible into developing a suitable level-set speed function so that an evolving contour exactly segments a cell/nuclei blob. We use image gradients, distance maps, multiple...
The ultimate aim of many live-cell fluorescence microscopy imaging experiments is the quantitative analysis of the spatial structure and temporal behavior of intracellular objects. This requires finding the precise geometrical correspondence between the time frames for each individual cell and performing intracellular segmentation. In a previous paper we have developed a powerful multi-level-set based...
This work presents a unified framework for whole cell segmentation of surface stained living cells from 3-D data sets of fluorescent images. Every step of the process is described, image acquisition, prefiltering, ridge enhancement, cell segmentation, and a segmentation evaluation. The segmentation results from two different automated approaches for segmentation are compared to manual segmentation...
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